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Harry Jarrett
Title Professor
Faculty Rank
Degree Ph.D.
Institution University of Texas at San Antonio
Department Chemistry
Clusters Other
1 UTSA Cir
City San Antonio
State TX
Postal Code 78249
Telephone (210) 458-7053
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  Signal transduction, and more specifically the role of Ca2+ and its receptor protein, calmodulin, in cellular regulation
We have shown that dystrophin, the protein product of the Duchenne muscular dystrophy gene locus, is a specific calmodulin-binding protein and discovered a signal transduction pathway, which links laminin-a-dystroglycan-ß-dystroglycan-dystrophin-syntrophin-Grb2-Sos1-Rac1-PAK1-MKK4/7-JNK, causing c-jun phosphorylation and activation.
This work is supported by National Institutes of Health grant 1R01AR051440.
Transcription factor purification and characterization
We first coupled DNA to HPLC silica and have made a significant contribution to the purification and characterization of transcription factors. Most recently we developed systematic oligonucleotide trapping, a method for purifying transcription factors to near homogeneity in a single step. Current experiments involve promoter trapping, a method which interfaces DNA-HPLC with proteomic techniques (MALDI-TOF and ESE-ion trap) to characterize the transcription complex of the c-jun promoter.
This work is supported by National Institutes of Health grant 1R01GM43609.

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  Research projects funded by the National Institutes of Health (NIH), the Centers for Disease Control (CDC), the Food and Drug Administration (FDA), and the Department of Veterans Affairs (VA)

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2013Polynucleotide High Performance Affinity Chromatography5R01GM043609-24
2012Polynucleotide High Performance Affinity Chromatography5R01GM043609-23
2011Polynucleotide High Performance Affinity Chromatography5R01GM043609-22
2010Muscle Cell Signaling5R01AR051440-05
2010Polynucleotide High Performance Affinity Chromatography2R01GM043609-21A2
2009Muscle Cell Signaling5R01AR051440-04
2008Polynucleotide High Performance Affinity Chromatography5R01GM043609-20
2008Muscle Cell Signaling5R01AR051440-03
2007Muscle Cell Signaling5R01AR051440-02
2007Polynucleotide High Performance Affinity Chromatography5R01GM043609-19
2006Polynucleotide High Performance Affinity Chromatography5R01GM043609-18
2006Muscle Cell Signaling1R01AR051440-01A2
2005Polynucleotide High Performance Affinity Chromatography7R01GM043609-17
2005Polynucleotide High Performance Affinity Chromatography2R01GM043609-16
2004Polynucleotide High Performance Affinity Chromatography5R01GM043609-15
2003Polynucleotide High Performance Affinity Chromatography5R01GM043609-14
2002Polynucleotide High Performance Affinity Chromatography5R01GM043609-13
2001Polynucleotide High Performance Affinity Chromatography2R01GM043609-12A1
2001Polynucleotide High Performance Affinity Chromatography3R01GM043609-12A1S1

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1. Jia Y, Jarrett HW. Method for trapping affinity chromatography of transcription factors using aldehyde-hydrazide coupling to agarose. Anal Biochem. 2015 Aug 01; 482:1-6.
2. Wang L, Jiao Y, Sun S, Jarrett HW, Sun D, Gu W. Gene network of a phosphoglycerate mutase in muscle wasting in mice. Cell Biol Int. 2015 Jun; 39(6):666-77.
3. Nagore LI, Jarrett HW. T(3): targeted proteomics of DNA-binding proteins. Anal Biochem. 2015 Apr 01; 474:8-15.
4. Jia Y, Nagore L, Jarrett H. Southwestern Blotting Assay. Methods Mol Biol. 2015; 1334:85-99.
5. Nagore LI, Zhou Y, Nadeau RJ, Jia Y, Jarrett HW. Promoter trapping method: transcription factor purification using human telomerase reverse transcriptase promoter. Proteome Sci. 2014; 12(1):53.
6. Jia Y, Larionov O, Jarrett HW. Coupling of deoxyribonucleic acid to solid supports using 3' terminal ribose incorporation. J Chromatogr A. 2014 Apr 25; 1339:73-9.
7. Hoffmann C, Zimmermann A, Hinney A, Volckmar AL, Jarrett HW, Fromme T, Klingenspor M. A novel SP1/SP3 dependent intronic enhancer governing transcription of the UCP3 gene in brown adipocytes. PLoS One. 2013; 8(12):e83426.
8. Nagore LI, Nadeau RJ, Guo Q, Jadhav YL, Jarrett HW, Haskins WE. Purification and characterization of transcription factors. Mass Spectrom Rev. 2013 Sep-Oct; 32(5):386-98.
9. Zhou Y, Jia Y, Jarrett HW. Asymmetric polymerase chain reaction provides alternatives for preparation of (GT)5-tailed duplex DNA promoter for promoter trapping. Anal Biochem. 2012 Aug 15; 427(2):133-8.
10. Jarrett HW. Proteomic methodologies to study transcription factor function. Methods Mol Biol. 2012; 786:315-34.
11. Zhou YW, Munoz J, Jiang D, Jarrett HW. Laminin-a1 LG4-5 domain binding to dystroglycan mediates muscle cell survival, growth, and the AP-1 and NF-?B transcription factors but also has adverse effects. Am J Physiol Cell Physiol. 2012 Mar 15; 302(6):C902-14.
12. Jiang D, Jia Y, Jarrett HW. Transcription factor proteomics: identification by a novel gel mobility shift-three-dimensional electrophoresis method coupled with southwestern blot and high-performance liquid chromatography-electrospray-mass spectrometry analysis. J Chromatogr A. 2011 Sep 28; 1218(39):7003-15.
13. Jiang D, Mummidi S, Ahuja SK, Jarrett HW. CCR5 promoter haplotype transcription complex characterization. J Health Care Poor Underserved. 2011; 22(4 Suppl):73-90.
14. Jia Y, Jiang D, Jarrett HW. Repeated probing of Southwestern blots using alkaline phosphatase stripping. J Chromatogr A. 2010 Nov 05; 1217(45):7177-81.
15. Munoz J, Zhou Y, Jarrett HW. LG4-5 domains of laminin-211 binds alpha-dystroglycan to allow myotube attachment and prevent anoikis. J Cell Physiol. 2010 Jan; 222(1):111-9.
16. Jiang S, Galindo MR, Jarrett HW. Purification and identification of a transcription factor, USF-2, binding to E-box element in the promoter of human telomerase reverse transcriptase (hTERT). Proteomics. 2010 Jan; 10(2):203-11.
17. Jiao Y, Jin X, Yan J, Jiao F, Li X, Roe BA, Jarrett HW, Gu W. An insertion of intracisternal A-particle retrotransposon in a novel member of the phosphoglycerate mutase family in the lew allele of mutant mice. Genes Genet Syst. 2009 Oct; 84(5):327-34.
18. Jiang D, Jarrett HW, Haskins WE. Methods for proteomic analysis of transcription factors. J Chromatogr A. 2009 Oct 09; 1216(41):6881-9.
19. Jiang D, Jia Y, Zhou Y, Jarrett HW. Two-dimensional southwestern blotting and characterization of transcription factors on-blot. J Proteome Res. 2009 Jul; 8(7):3693-701.
20. Xiong Y, Zhou Y, Jarrett HW. Dystrophin glycoprotein complex-associated Gbetagamma subunits activate phosphatidylinositol-3-kinase/Akt signaling in skeletal muscle in a laminin-dependent manner. J Cell Physiol. 2009 May; 219(2):402-14.
21. Jiang D, Zhou Y, Moxley RA, Jarrett HW. Purification and identification of positive regulators binding to a novel element in the c-Jun promoter. Biochemistry. 2008 Sep 02; 47(35):9318-34.
22. Panda M, Jiang D, Jarrett HW. Trapping of transcription factors with symmetrical DNA using thiol-disulfide exchange chemistry. J Chromatogr A. 2008 Aug 15; 1202(1):75-82.
23. Zhou Y, Jiang D, Thomason DB, Jarrett HW. Laminin-induced activation of Rac1 and JNKp46 is initiated by Src family kinases and mimics the effects of skeletal muscle contraction. Biochemistry. 2007 Dec 25; 46(51):14907-16.
24. Jiang D, Moxley RA, Jarrett HW. Promoter trapping of c-jun promoter-binding transcription factors. J Chromatogr A. 2006 Nov 10; 1133(1-2):83-94.
25. Zhou YW, Thomason DB, Gullberg D, Jarrett HW. Binding of laminin alpha1-chain LG4-5 domain to alpha-dystroglycan causes tyrosine phosphorylation of syntrophin to initiate Rac1 signaling. Biochemistry. 2006 Feb 21; 45(7):2042-52.
26. Nagore LI, Mitra S, Jiang D, Jiang S, Zhou Y, Loranc M, Jarrett HW. Cyanogen bromide-activated coupling: DNA catalytic chromatography purification of EcoRI endonuclease. Nat Protoc. 2006; 1(6):2909-15.
27. Mitra S, Jarrett HW, Jurado LA. High-performance catalytic chromatography. The adapter approach. J Chromatogr A. 2005 May 27; 1076(1-2):71-82.
28. Moxley RA, Jarrett HW. Oligonucleotide trapping method for transcription factor purification systematic optimization using electrophoretic mobility shift assay. J Chromatogr A. 2005 Apr 08; 1070(1-2):23-34.
29. Zhou YW, Oak SA, Senogles SE, Jarrett HW. Laminin-alpha1 globular domains 3 and 4 induce heterotrimeric G protein binding to alpha-syntrophin's PDZ domain and alter intracellular Ca2+ in muscle. Am J Physiol Cell Physiol. 2005 Feb; 288(2):C377-88.
30. Robinson FD, Moxley RA, Jarrett HW. Effect of the detergent Tween-20 on the DNA affinity chromatography of Gal4, C/EBPalpha, and lac repressor with observations on column regeneration. J Chromatogr A. 2004 Jan 23; 1024(1-2):71-8.
31. Moxley RA, Jarrett HW, Mitra S. Methods for transcription factor separation. J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Nov 25; 797(1-2):269-88.
32. Matsuoka TA, Zhao L, Artner I, Jarrett HW, Friedman D, Means A, Stein R. Members of the large Maf transcription family regulate insulin gene transcription in islet beta cells. Mol Cell Biol. 2003 Sep; 23(17):6049-62.
33. Oak SA, Zhou YW, Jarrett HW. Skeletal muscle signaling pathway through the dystrophin glycoprotein complex and Rac1. J Biol Chem. 2003 Oct 10; 278(41):39287-95.
34. Jurado LA, Jarrett HW. In flow activation of diol-silica with cyanogen bromide and triethylamine for preparing high-performance affinity chromatographic columns. J Chromatogr A. 2003 Jan 10; 984(1):9-17.
35. Jurado LA, Mosley J, Jarrett HW. Cyanogen bromide activation and coupling of ligands to diol-containing silica for high-performance affinity chromatography optimization of conditions. J Chromatogr A. 2002 Sep 20; 971(1-2):95-104.
36. Gadgil H, Jarrett HW. Oligonucleotide trapping method for purification of transcription factors. J Chromatogr A. 2002 Aug 09; 966(1-2):99-110.
37. Chockalingam PS, Cholera R, Oak SA, Zheng Y, Jarrett HW, Thomason DB. Dystrophin-glycoprotein complex and Ras and Rho GTPase signaling are altered in muscle atrophy. Am J Physiol Cell Physiol. 2002 Aug; 283(2):C500-11.
38. Chockalingam PS, Gadgil H, Jarrett HW. DNA-support coupling for transcription factor purification. Comparison of aldehyde, cyanogen bromide and N-hydroxysuccinimide chemistries. J Chromatogr A. 2002 Jan 04; 942(1-2):167-75.
39. Gadgil H, Oak SA, Jarrett HW. Affinity purification of DNA-binding proteins. J Biochem Biophys Methods. 2001 Oct 30; 49(1-3):607-24.
40. Chockalingam PS, Jurado LA, Jarrett HW. DNA affinity chromatography. Mol Biotechnol. 2001 Oct; 19(2):189-99.
41. Oak SA, Russo K, Petrucci TC, Jarrett HW. Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Biochemistry. 2001 Sep 18; 40(37):11270-8.
42. Gadgil H, Taylor WL, Jarrett HW. Comparative studies on discrete and concatemeric DNA-sepharose columns for purification of transcription factors. J Chromatogr A. 2001 May 11; 917(1-2):43-53.
43. Gadgil H, Jurado LA, Jarrett HW. DNA affinity chromatography of transcription factors. Anal Biochem. 2001 Mar; 290(2):147-78.
44. Gadgil H, Taylor WL, Jarrett HW. Bi-column method for purification of transcription factors. J Chromatogr A. 2001 Jan 05; 905(1-2):133-9.
45. Oak SA, Jarrett HW. Oligomerization of mouse alpha 1-syntrophin and self-association of its pleckstrin homology domain 1 containing sequences. Biochemistry. 2000 Aug 01; 39(30):8870-7.
46. Jurado LA, Drummond JT, Jarrett HW. Catalytic chromatography. Anal Biochem. 2000 Jun 15; 282(1):39-45.
47. Jarrett HW. Temperature dependence of DNA affinity chromatography of transcription factors. Anal Biochem. 2000 Mar 15; 279(2):209-17.
48. Chockalingam PS, Jurado LA, Robinson FD, Jarrett HW. DNA affinity chromatography. Methods Mol Biol. 2000; 147:141-53.
49. Madhavan R, Jarrett HW. Phosphorylation of dystrophin and alpha-syntrophin by Ca(2+)-calmodulin dependent protein kinase II. Biochim Biophys Acta. 1999 Oct 12; 1434(2):260-74.
50. Robinson FD, Gadgil H, Jarrett HW. Comparative studies on chemically and enzymatically coupled DNA-Sepharose columns for purification of a lac repressor chimeric fusion protein. J Chromatogr A. 1999 Jul 23; 849(2):403-12.
51. Gadgil H, Jarrett HW. Heparin elution of transcription factors from DNA-Sepharose columns. J Chromatogr A. 1999 Jul 02; 848(1-2):131-8.
52. Jurado LA, Chockalingam PS, Jarrett HW. Apocalmodulin. Physiol Rev. 1999 Jul; 79(3):661-82.
53. Chockalingam PS, Gee SH, Jarrett HW. Pleckstrin homology domain 1 of mouse alpha 1-syntrophin binds phosphatidylinositol 4,5-bisphosphate. Biochemistry. 1999 Apr 27; 38(17):5596-602.
54. Jarrett HW, Taylor WL. Transcription factor-green fluorescent protein chimeric fusion proteins and their use in studies of DNA affinity chromatography. J Chromatogr A. 1998 Apr 17; 803(1-2):131-9.
55. Newbell BJ, Anderson JT, Jarrett HW. Ca2+-calmodulin binding to mouse alpha1 syntrophin: syntrophin is also a Ca2+-binding protein. Biochemistry. 1997 Feb 11; 36(6):1295-305.
56. Jarrett HW. Hybrid selection with cDNA-silica. J Chromatogr A. 1996 Aug 23; 742(1-2):87-94.
57. Anderson JT, Rogers RP, Jarrett HW. Ca2+-calmodulin binds to the carboxyl-terminal domain of dystrophin. J Biol Chem. 1996 Mar 22; 271(12):6605-10.
58. Madhavan R, Jarrett HW. Interactions between dystrophin glycoprotein complex proteins. Biochemistry. 1995 Sep 26; 34(38):12204-9.
59. Jarrett HW. Preparation of cDNA-silica using reverse transcriptase and its DNA sequence determination. J Chromatogr A. 1995 Jul 28; 708(1):13-8.
60. Jarrett HW, Foster JL. Alternate binding of actin and calmodulin to multiple sites on dystrophin. J Biol Chem. 1995 Mar 10; 270(10):5578-86.
61. DiRusso C, Rogers RP, Jarrett HW. Novel DNA-Sepharose purification of the FadR transcription factor. J Chromatogr A. 1994 Aug 12; 677(1):45-52.
62. Madhavan R, Jarrett HW. Calmodulin-activated phosphorylation of dystrophin. Biochemistry. 1994 May 17; 33(19):5797-804.
63. Paudel HK, Xu YH, Jarrett HW, Carlson GM. The model calmodulin-binding peptide melittin inhibits phosphorylase kinase by interacting with its catalytic center. Biochemistry. 1993 Nov 09; 32(44):11865-72.
64. Lee HG, Jarrett HW. Chromatographic stability of glucose-silica. J Chromatogr A. 1993 Oct 29; 653(1):130-4.
65. Jarrett HW. Affinity chromatography with nucleic acid polymers. J Chromatogr. 1993 Aug 25; 618(1-2):315-39.
66. Madhavan R, Massom LR, Jarrett HW. Calmodulin specifically binds three proteins of the dystrophin-glycoprotein complex. Biochem Biophys Res Commun. 1992 Jun 15; 185(2):753-9.
67. Massom LR, Jarrett HW. High-performance affinity chromatography of DNA. II. Porosity effects. J Chromatogr. 1992 May 29; 600(2):221-8.
68. Solomon LR, Massom LR, Jarrett HW. Enzymatic syntheses of DNA-silicas using DNA polymerase. Anal Biochem. 1992 May 15; 203(1):58-69.
69. Goss TA, Bard M, Jarrett HW. High-performance affinity chromatography of messenger RNA. J Chromatogr. 1991 Dec 27; 588(1-2):157-64.
70. Massom LR, Lukas TJ, Persechini A, Kretsinger RH, Watterson DM, Jarrett HW. Trifluoperazine binding to mutant calmodulins. Biochemistry. 1991 Jan 22; 30(3):663-7.
71. Jarrett HW, Madhavan R. Calmodulin-binding proteins also have a calmodulin-like binding site within their structure. The flip-flop model. J Biol Chem. 1991 Jan 05; 266(1):362-71.
72. Lee HG, Jarrett HW. Glucose-silica, an improved medium for high-pressure gel filtration chromatography. J Chromatogr. 1990 Jul 06; 511:69-78.
73. Goss TA, Bard M, Jarrett HW. High-performance affinity chromatography of DNA. J Chromatogr. 1990 Jun 01; 508(2):279-87.
74. Massom L, Lee H, Jarrett HW. Trifluoperazine binding to porcine brain calmodulin and skeletal muscle troponin C. Biochemistry. 1990 Jan 23; 29(3):671-81.
75. Massom LR, Jarrett HW. Purification of argininosuccinase by high-pressure immunoaffinity chromatography on monoclonal anti-argininosuccinase-silica. J Chromatogr. 1989 Nov 17; 482(1):252-7.
76. Persechini A, Blumenthal DK, Jarrett HW, Klee CB, Hardy DO, Kretsinger RH. The effects of deletions in the central helix of calmodulin on enzyme activation and peptide binding. J Biol Chem. 1989 May 15; 264(14):8052-8.
77. Reysz LJ, Carroll AG, Jarrett HW. An automated assay for adenylate cyclase using reversed-phase high-performance liquid chromatography. Anal Biochem. 1987 Oct; 166(1):107-12.
78. Foster WS, Jarrett HW. Melittin-silica, a high-pressure affinity chromatography resin for calmodulin. J Chromatogr. 1987 Aug 21; 403:99-107.
79. Jarrett HW. High-pressure affinity chromatography of calmodulin on a phenothiazine-silica. J Chromatogr. 1986 Aug 29; 363(2):456-61.
80. Jarrett HW, Cooksy KD, Ellis B, Anderson JM. The separation of o-phthalaldehyde derivatives of amino acids by reversed-phase chromatography on octylsilica columns. Anal Biochem. 1986 Feb 15; 153(1):189-98.
81. Harmon AC, Jarrett HW, Cormier MJ. An enzymatic assay for calmodulins based on plant NAD kinase activity. Anal Biochem. 1984 Aug 15; 141(1):168-78.
82. Jarrett HW, Charbonneau H, Anderson JM, McCann RO, Cormier MJ. Plant calmodulin and the regulation of NAD kinase. Ann N Y Acad Sci. 1980; 356:119-29.

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